INTRODUCTION:
An atomic absorption spectrometer is a device
that is used to analyze the number of metals in a sample of liquid. It is a
susceptible device that can detect the sample in minimal concentration that is
parts per million (ppm) or parts per billion (ppb). The atomicabsorption spectrometer for sale is there on the internet, but all you
have to see is, how much the spectrometer is sensitive and accurate. In atomic
absorption spectrometry, there is a specific beam of light which is passed
through the crystal structure of an atom, the amount of energy is calculated in
the form of a photon (small packets of energy) and then detected by the
detector.
PRINCIPLE:
Atomic absorption spectroscopy works on the
principle of absorption of radiation by free atoms; the more the free atoms
present in the atomic structure, the more light is absorbed.
INSTRUMENTATIONS:
The AAS uses atomizer, which converts the
sample in vapor form. For atomization, two types of atomizers are used that is
flame and Electrothermal source (e.g., graphite tube). The sample is then
irradiated with a beam of radiation. As every radiations are absorbed after
absorption by the sample, but we only need the specific radiation, so we use a
monochromator, which separates the particular wavelength of light. In the end,
there is a detector attached to the device which detects the radiation.
WORKING:
There are two steps involved in the
functioning of atomic absorption spectrometry:
- Atomization of the sample
- Absorption of light by the sample and emission of radiations.
After the atomization of the sample, the atoms
absorb light by a light source (e.g., hollow cathode tube) the particles which
are dispersed absorbs light. After the absorption of light, the atoms in
samples from the ground state are transitioned to an excited state. When they
come back to the ground state, they emit energy in the form of radiation, which
is detected by the detector finally.
The method of AAS is selective and sensitive
both. As not every element in the samples absorbs light; hence it is useful for detecting the
purity of compounds.
APPLICATIONS:
There are several applications of AAS:
- It is used in analytical chemistry to check the analysis and check the purity of compounds.
- It is used in the pharmaceutical industry for checking the compounds. And check the chemical structure of the compounds too.
- Furthermore, it is used to check the purity of the drinking water and the grade of food quality in food industries.
The spectrophotometers are needed to be
validated and calibrated before their use in order to get precision and
reproducible results.
So to conclude, it can be said that the atomic
absorption spectrometer is an essential device that has many uses and
applications in the pharmaceutical industry and other industries too. So
whenever anybody wishes to buy a spectrometer, they should take any analyst or
the person who knows about it to get an accurate and precise spectrophotometer.
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